Deuterated oxazines are bright near-infrared fluorophores for mitochondrial imaging and single molecule spectroscopy
Authors
- B. Gatin-Fraudet
- J. Seifert
- K. Sarach
- C.H. Olesen
- R. Birke
- S. Milles
- M. Birol
- M. Lehmann
- J. Broichhagen
Journal
- Chemical Communications
Citation
- Chem Commun 61 (17): 3540-3543
Abstract
Bright near-infrared fluorophores are in demand for microscopy. We showcase a deuterated oxazine being 23% brighter vs. ATTO700. With a longer lifetime of 1.85 nanoseconds, we find the best-in-class SulfoOxazine700-d10 to stain mitochondria for confocal microscopy, and demonstrate unaffected diffusion properties in single molecule fluorescence correlation spectroscopy.